HPLC梯度洗脱测定痔康泰纳米粒中大黄蒽醌成分的总含量

Content determination of total anthraquinones in Zhi Kangtai nanoparticles by HPLC gradient elution

目的建立同时测定痔康泰（ZKT）纳米粒中5种大黄蒽醌成分总量的方法。方法采用RP—HPLC法，色谱柱为Agilent Zorbax C18（250mm×4．6mm，5um）柱，流动相为甲醇-0．1％磷酸梯度洗脱，流速为1．0ml·min^-1，柱温为30℃，检测波长为254nm。结果各成分达到基线分离，峰形良好。芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的线性范围分别为8．76～87．60（r=0．9999）、7．85—78．50（r=0．9999）、7．48～74．80（r=0．9999）、8．24～82．40（r=0．9999）、5．58～55．80ug·ml^-1（r=0．9998）。平均加样回收率分别为99．76％（RSD=1．92％）、101．53％（RSD=1．73％）、98．47％（RSD：1．59％）、103．62％（RSD=1．75％）、102．94％（RSD=1．63％）。结论所建方法简便可行、重复性好，可用于评价和控制ZKT纳米粒的质量。

OBJECTIVE To establish a method for the determination of total content of five rhubarb anthraquinones in Zhi Kangtai nanoparticles. METHODS RP - HPLC was adopted. Rhubarb anthraquinones were simultaneously separated and determined with an Agilent Zorbax C18 column（250 mm ×4.6 mm,5 um）by gradient elution using methanol-0. 1% phosphoric acid solution as the mobile phase. The flow rate was 1.0 ml. min^-1 and the detective wavelength was set at 254 nm with column temperature of 30℃. RESULTS The five rhubarb anthraquinones were completely separated and were not interfered by other components. Every rhubarb anthraquinone＇ s linearity range, correlation coefficient and average recoveries were showed respectively as follows ： linearity range of aloe - emodin：8.76 - 87.6 ug·ml^-1 （ r = 0. 9999 ）, average recovery 99.76% with RSD of 1.92% ; linearity range of rhein：7.85 - 78. 5 ug. ml^-1 （ r = 0. 9999）, average recovery 101.53 % with RSD of 1.73 % ; linearity range of emodin ： 7.48 - 74.8ug. ml^-1 （ r = 0. 9999）, average recovery 98.47 % with RSD of 1.59 % ; linearity range of chrysophanol ： 8.24 - 82.4ug. ml^-1（ r = 0.9999）, average recovery 103.62% with RSD of 1.75% ;linearity range of physcion ：5. 58 - 55.8ug. ml^-1 （r =0. 9998） ,average recovery 102.94 % with RSD of 1.63%. CONCLUSION This method is simple, feasible and reproducible. It can be used for the quality control of Zhi Kang tai nanoparticles.