HPLC测定东方肉穗草中的槲皮素

Determination of quercetin in Sarcopyramis bodinieri var.delicata by HPLC

目的建立测定东方肉穗草中槲皮素含量的方法。方法采用RP—HPLC法，色谱柱为Shimadzu Shimpack VP C18柱（150mm×46mm，5um），流动相为甲醇-0．1％磷酸（45：55），流速为1．0ml·min^-1，检测波长为365nm，柱温为40℃。结果槲皮素0．025～0．400mg·ml^-1与峰面积呈良好的线性关系，回归方程为：Y=7．74×10^-7X-2．69×10^5（r=0．9998），平均回收率为99．8％，RSD=0．83％（n=5）。结论所建方法快速、简便，重复性和稳定性均好，能准确地测定东方肉穗草中槲皮素的含量。

OBJECTIVE To establish a method for determining the content of quercetin in Sarcopyramis bodinieri var. delicata by HPLC. METHODS The Shimadzu Shimpack VP C18 （150 mm × 46 mm ,5 um ） column with methanol -water- phosphoric acid （45 ： 55 ） as the mobile phase was used. The flow rate was 1. 0 ml. min - 1 and the detective wavelength was set at 365 nm. The column temperature was set at 40 ℃. RESULTS The calibration curve was linear in the range of 0.025 - 0.40 mg·ml^-1 , Y = 7.74 × 107 X - 2. 69 × 10^5 （ r = 0. 9998 ）. The average recovery was 99.8% with the RSD of 0.83%. CONCLUSION The method is simple, accurate, reproducible and can be used for the quality control of Sarcopyramis bodinieri vat. delicata.