HPLC测定沙棘油中的α-生育酚

Determination of α-tocopherol in Hippophae oil by HPLC

目的建立一种简便的测定沙棘油中α-生育酚含量的方法。方法以无水乙醇提取沙棘油中的α-生育酚，用HPLC测定，固定相为C18（15mm×4．6mm，5um）色谱柱，流动相为甲醇-水（95：5），流速为1．0ml·min^-1，检测波长为290nm。结果α-生育酚与其他醇溶性成分分离良好；其浓度2．4～22．0mg·L^-1与峰面积的线性关系良好（r=0．9999）；平均回收率为96．81％。结论与传统皂化法比较，所建方法更为简便、快速和准确。

OBJECTIVE To establish a simple HPLC method for the determination of α - tocopherol in Hippophae oil. METHODS Absolute ethanol was used to extract α - tocopherol from Hippophae oil and the content was determined by HPLC. The separation was performed on a Cls column （15 mm ×4.6 mm,5 um）. The mobile phase consisted of methanol -water （95 ： 5） with the flow rate of 1.0 ml·min^-1. The detection wavelength was set at 290 nm. RESULTS α -Tocopherol could be well separated from other components soluble in ethanol. The standard curve was linear in the concentration range from 2.4 to 22.0 rag. L- 1 （ r = 0.9999）. The mean recovery was 96.81%. CONCLUSION The established method is simpler, quicker and more accurate than the traditional method of alkali saponifing. It is suitable for assaying the content of α - tocopherol in Hippophae oil.