异噻唑酮对标本防腐和固定的观察

ANTISEPTIC AND FIXATIVE OBSERVATIONS OF ISOTHIAZOLONE EFFECT ON THE CADAVERS

用0.1％的异噻唑酮水溶液,对尸体标本作防腐观察3年,结果表明:溶液清亮,标本颜色保存良好,结构层次清楚,无霉菌生长。光镜下观察动物防腐标本的组织切片,见结构清楚,细胞完整。抑菌试验表明,0.05～0.1％的水溶液对常见污染细菌及霉菌均有良好的抑制作用;对尸体上取下的黄曲霉菌等,抑制效果可靠。异噻唑酮水溶液稳定,半分解期为93.28个月,按0.1％水溶液配制防腐,抑霉作用可保持7年有效。其水溶液无色、无味、无刺激。毒性试验LD_(50)为175mg/kg;Ames法突变试验阴性;致畸试验不引起鼠胎骨畸形。以它代替甲醛作为标本防腐剂,有更好的实用价值。

Isothiazolone exhibit a broad-spectral antiseptic actions. The formalin fixed cadavers preserved in 0.1％ isothiazolone aqueous solution for 3 years, revealed that the solution still kept clear, and the colour of the cadavers was well retained, and the gross structure of the cadavers was in good condition. Bacteriostatic test indicated that 0.05-0.1％ isothiazolone solution was highly effective in inhibiting common septic bacteria and fungi, and was reliably effective in inhibiting the aspergillus flavus taken from the surface of the midewy cadavers. The chemical property of the isothiazolone solution was stable, and its T_(1/2) was 93.28 months, and the bacteriostatic effect might keep for 7 years. The solution was colourless, odorless and nonirritant. Toxicity test revealed its LD_(50) was 175mg/kg, and the Ames' mutation test showed a negative result, and the teratologic study indicated that it did not induce any skeletal malformations in neonatal rats. This study suggest that 0.1％ isothiazolone may be used as a preservative to substitute for formaldehyde.