胰腺实性假乳头状瘤病理与免疫组化分析

Immunohistochemical study of solid pseudopapillary tumors of pancreas

目的:运用免疫组化技术探讨胰腺实性假乳头状瘤(solid pseudopapillary tumors of pancreas,SPT)的病理形态特征及组织起源。方法:免疫组化EnVision法检测β-cate-nin、E-cad、Fli-1、CD56、α1-AT、Vim、NSE、CD10、PR、Syn、CgA及CKpan在35例SPT组织中的表达。结果:β-catenin核(质)阳性表达为86%、E-cad3%、Fli-141%、CD5654%、α1-AT94%、Vim100%、NSE89%、CD1086%、PR60%、Syn51%、CgA34%及CKpan31%。β-catenin与E-cad的表达差异有统计学意义,P<0.05。结论:β-catenin异常核表达与E-cad表达缺失存在相关性,两者在SPT特殊的假乳头形态的形成中可能起重要作用;多种抗体表达支持SPT起源于多潜能干细胞的假说。

OBJECTIVE： To study the histopathological features and histogenesis of solid pseudopapillary tumors of pancreas （SPT） using the immunohistochemical method. METHODS： EnVision immunostaining was used to test the expressions of β-catenin, E-cadherin, Fli 1, CD56, αl-AT, Vim, NSE, CD10, PR, Syn, CgA and CKpan on SPT. RESULTS： Immunohistochemical ly, the tumors were positive for β-catenin nuclear/cytoplasma （86G）, E-cadherin （3G）, Flil （41%）, CD56 （54G）, αl-AT （94%）, Vim （100G）, NSE （89G）, CD10 （86G）, PR （60G）, Syn （51G）, CgA （34%） and Ckpan （31%）. The expressions of β-catenin and E eadherin were significantly different, P〈0.05. CONCLUSIONS： There is a correlation between nuclear expression of β-eatenin and loss expression of E-cadherin, both of which may play important roles in the formation of a specific pseudopapillary pattern of SPT. The expressions of various antibodies favor the hypotheses that SPT originates from multipotent primordial ceils.