PCR-SSP法用于Dombrock血型Do^a/Do^b的基因分型

Study on dombrock blood group Do^a and Do^b genotyping by PCR-SSP technique

目的:了解Dombrock血型Doa和Dob基因在汉族人群中的分布。方法:采用PCR-SSP技术对Dombrock血型进行Doa和Dob基因分型。结果:248例Dombrock血型Doa的基因频率为0.0867,Dob的基因频率为0.9133。结论:248例Dombrock血型Doa及Dob的基因频率分别为0.0867和0.9133。PCR-SSP技术可以较好的对Dombrock血型进行DO基因分型。

Objective：To discuss the Dombrock blood group Do^a and Do^b and the difference between the RhD^- and RhD^＋. Method： Molecular genetic Doa and Dob are typied by PCR-SSP. Result：Do^a Genetic frequency is 0. 0867 in 248 samples, Do^b is 0. 9133. Conclusion：PCR-SSP is an easy-to-handle method to typing DO blood group ;Do^a and Do^b gene frequency are 0. 0867 and 0. 9133 in 248 samples.