再生神经单根纤维中轴突和髓鞘构筑的图像分析

IMAGE ANALYSIS OF ARCHITECTURES OF AXON AND MYELIN SHEATH IN TEASED SINGLE NERVE FIBER DURING NERVE REGENERATION

为进一步认识神经再生的规律,由夹伤的大鼠坐骨神经分离出单根神经纤维,用图像分析技术测定了98天再生过程中轴突与髓鞘的直径(厚度)、面积和体积等项构筑参数。发现再生早期受损伤的神经纤维生长较快,56～98天生长较缓。在98天的再生过程中,轴突和髓鞘的生长基本平行,从形态计量学的角度上,反映了神经再生中轴突与髓鞘的密切关系。这也是我们提出的神经再生受神经元和非神经元性因素共同调控的进一步佐证。

The regenerating sciatic nerve of rat was teased into single nerve fibers at various intervals in 98 days after crushing. By image analysis, it was found that the values of architectural parameters of axon and myelin sheath, such as axonal diameter, myelin sheath thickess, their surface area and volume all increased apparently before 56 days, no any significant change was revealed between 56～98 days, and did not reach the level of each individual parent stump even upto 98 th day. In comparison of the architectural changes between axon and myelin sheath further, a parallel development in time course was demonstrated. The result not only indicated that a close relationship existed between axon and myelin sheath during regeneration, but also provided a further evidence that nerve regeneration was cooperated by the interation of both neuronal and nonneuronal regulators, which has been reported previously.