大鼠抗-HRP单克隆抗体的制备及应用的研究

PREPARATION AND APPLICATION OF A RAT ANTI-HRP MONOCLONAL ANTIBODY

本文采用大鼠杂交瘤体系制备了大鼠抗HRP McAb,并以此与HRP制成McPAP复合物。本文的结果表明,我们制成的McPAP复合物不仅可用于免疫组织化学法,也可用于ELISA法。均获得强的染色反应而背景清晰,并对McPAP与酶标记抗体的灵敏度进行了比较。

The rat anti-HRP McAbs were prepared with rat-rat hybridoma and the rat McPAP complex was made by conjugating this McAb with HRP. The results of the present work indicated that the rat MePAP can be used for detection of antibodyantigen reaction by immunohistochemical techniques and ELISA. In both assay it gave strong staining reaction for positive antigen and with background staining appeared very pale. The sensitivity of rat McPAP and HRP labelling rabbit anti-rat IgG has been compared.