豚鼠粘液腺组织化学的观察

HISTOCHEMICAL OBSERVATION ON MUCOUS GLANDS IN GUINEA PIGS

本实验利用酶标凝集素和组织化学的方法,对豚鼠舌下腺、幽门腺、十二指肠腺、大肠腺和主胰管腺及其杯状细胞等进行了研究。发现舌下腺和幽门腺含有大量带有唾液酸的粘多糖,而其余3种腺体则含有大量的硫酸粘多糖。此外,这些腺体对PNA和DBA的反应也有很大不同,如舌下腺和大肠腺均不与PNA和DBA反应。幽门腺、十二指肠腺以及主胰管腺虽与DBA呈强阳性反应,但对PNA反应则不同。分布在十二指肠、大肠和主胰管的杯状细胞,尽管形态相似,但对PAS和AB的反应,以及与凝集素的结合有显著差别,甚至在同一器官内不同位置的杯状细胞,也出现不同反应。各种粘液腺和同一器官的不同部位的杯状细胞分泌物中的粘多糖不同。

The mucous glands (sublingual gland, pyloric gland, main pancreatic duct gland, duodenum gland and large intestinal gland) have been studied-by means of labelled lectins and histochemical methods. The results indicated that a large amount of mucopolysaccharide with sailic acid are found in sublingual glands and pyloric glands, while a large amount of sulfate mucopolysaccharide are observed in the other three glands. In addition, the reactivity of DBA and PNA in mucous glands are different, for example, the sublingual and large intestinal glands are negative for DBA and PNA staining. On the contrary, the pyloric glands, duodenum glands as well as main pancreatic duct glands are heavily stained with DBA, but various between the mucous glands stained with PNA. The goblet cells in duodenum, large intestine and main pancreatic duct are similar in their morphology, but the reactions of PAS, AB and lectins are different between them, even so in the same organ, the staining pattern also differ from their locations. The mucous glands and goblet cells contained different mucopolysaccharides, which might be concerr/ed with different functions in different organs.