急性戊型肝炎患者血清病毒抗原检测的临床意义

Detection of HEV Antigen in Patients with Acute Hepatitis E Infection

目的:探讨戊型肝炎抗原诊断试剂在临床诊断戊型肝炎病毒感染中的作用。方法:应用酶联免疫吸附试验方法检测戊型肝炎病毒抗原(HEV-Ag)试剂,对387例急性戊型肝炎患者血清标本进行检测,同时进行HEV RNA、抗HEV-IgM、抗HEV-IgG检测。结果:387例急性戊型肝炎患者血清HEV-Ag 140例(36.18%)阳性;单纯抗HEV-IgM阳性组的HEV-Ag阳性率最高;12例患者早期血清HEV-Ag阳性,随访过程中抗HEV-IgM与抗HEV-IgG阳转。结论:通过HEV-Ag检测,可以提高戊型肝炎病毒感染的诊断水平。

Objective：To discuss the clinical significance with using the diagnostic reagent of HEV-Ag. Methods： Three hundred and eighty seven sera from the patients with acute hepatitis E were detected by enzyme immunoassay （ELISA） for HEV-Ag,anti-HEV IgM and IgG and real time fluorescent RT-PCR for HEVRNA. Results：The serum samples from 140（36. 18;）of 387 patients were positive for HEV-Ag. Sera of anti-HEV lgM positive alone were the highest positive rate of HEV-Ag. 12 patients were positive for HEV-Ag and HEV-RNA in the early time of infection. Conclusion：Detection of HEV-Ag can raise the early diagnosis of HEV.