三氧化二砷对白血病HL-60细胞株的凋亡作用

Effect of Arsenic Trioxide on Leukemia Cell Line HL-60

目的：探讨三氧化二砷对HL-60的诱导凋亡作用。方法：琼脂糖电泳检测DNA条带;Hoecst 33258染色后荧光显微镜观察细胞核的变化。用流式细胞仪检测细胞凋亡率。结果：10μmol/L三氧化二砷处理HL-60细胞24~48 h可见到DNA出现梯形条带;处理24~48 h HL-60细胞核呈现细胞核浓缩和核碎裂现象。流式细胞仪测定12、24、和48 h的细胞凋亡率分别为（8.5±2.1）%、（12.8±3.4）%及（21.4±5.8）%,而培养48 h的HL-60细胞自然凋亡率仅为（1.5±0.5）%（P〈0.05）。结论：三氧化二砷可诱导HL-60细胞凋亡。

Objective：To explore the effect of arsenic trioxide on cell apoptosis in HL-60 cells. Methods：DNA（deoxyribonucleic acid） line was detected by gelose electrophresis. Changes of cell nucleus stained by Hoeest 33258 in HL-60 ceils treated by arsenic trioxide were studied by fluorescence microscope. The apoptosis were detected by flow cytome- try. Results ：DNA fragment appeared after HL-60 cells exposed to 10 μmol/L arsenic trioxide from 24 to 48 h ;cell nucleus were concentrated and fragmented after HL-60 cells exposed to 10 μmol/L arsenic trioxide for 48 h. Treated with 10 /;mol/L arsenic trioxide for 12hours,24 hours and 48 h,the apoptotic rate was （8.5±2.1）% ,（12.8±3.4）% and （21.4 ± 5.8） %, respectively, but the apoptotic rate in the negative control group only （1.5 ± 0, 5） % （P〈0. 05）. Conclusion ： Arsenic trioxide can induce apoptosis in HL-60 cells.