Oatp1和Oct1在大鼠下颌骨来源成骨细胞中的表达

Expression of Oatp1 and Oct1 in osteoblasts from rat mandible

目的:通过检测大鼠下颌骨成骨细胞中Oatp1和Oct1蛋白的表达,观察其在成骨细胞中的分布,探讨Oatp1和Oct1在成骨细胞跨膜转运中的作用及意义。方法:分离培养大鼠下颌骨成骨细胞并鉴定,应用免疫细胞化学及Western-Blot的方法检测大鼠下颌骨来源成骨细胞中Oatp1和Oct1蛋白的表达。结果:组织块法可成功培养大鼠下颌骨成骨细胞,Oatp1和Oct1在成骨细胞中有广泛表达,且主要分布在胞膜和胞质中。结论:大鼠下颌骨来源的成骨细胞可广泛表达Oatp1和Oct1,为研究药物在成骨细胞中的跨膜转运提供了基础。

Objective： To observe the distribution of Oatp 1 and Oct l in osteoblasts and to investigate the effect of Oatp 1 and Octl on the transmembrane transport of drug in osteoblasts by detecting the expression of Oatpl and Octl in osteoblasts from rat mandible. Methods： The primary cells were isolated from rat mandible and cultured by tissue explant culture technique. Morphological analysis and ALP, collagen I and alizarin red staining method were used to characterize the cell lineage. Then the expression of Oatpl and Octl were detected in osteoblasts by immunocytochemistry and Western-Blot. Results： The osteoblasts from rat mandible were cultured and passaged successfully. Oatpl and Octl were expressed extensively in osteoblasts, and mainly located in cell membrane and cytoplasm. Conclusion： Oatpl and Octl were expressed extensively in osteoblasts, which supplied the foundation for the transmembrane transport of clinical drug in osteoblasts.