摘要
目的:通过检测大鼠下颌骨成骨细胞中Oatp1和Oct1蛋白的表达,观察其在成骨细胞中的分布,探讨Oatp1和Oct1在成骨细胞跨膜转运中的作用及意义。方法:分离培养大鼠下颌骨成骨细胞并鉴定,应用免疫细胞化学及Western-Blot的方法检测大鼠下颌骨来源成骨细胞中Oatp1和Oct1蛋白的表达。结果:组织块法可成功培养大鼠下颌骨成骨细胞,Oatp1和Oct1在成骨细胞中有广泛表达,且主要分布在胞膜和胞质中。结论:大鼠下颌骨来源的成骨细胞可广泛表达Oatp1和Oct1,为研究药物在成骨细胞中的跨膜转运提供了基础。
Objective: To observe the distribution of Oatp 1 and Oct l in osteoblasts and to investigate the effect of Oatp 1 and Octl on the transmembrane transport of drug in osteoblasts by detecting the expression of Oatpl and Octl in osteoblasts from rat mandible. Methods: The primary cells were isolated from rat mandible and cultured by tissue explant culture technique. Morphological analysis and ALP, collagen I and alizarin red staining method were used to characterize the cell lineage. Then the expression of Oatpl and Octl were detected in osteoblasts by immunocytochemistry and Western-Blot. Results: The osteoblasts from rat mandible were cultured and passaged successfully. Oatpl and Octl were expressed extensively in osteoblasts, and mainly located in cell membrane and cytoplasm. Conclusion: Oatpl and Octl were expressed extensively in osteoblasts, which supplied the foundation for the transmembrane transport of clinical drug in osteoblasts.
出处
《口腔颌面修复学杂志》
2009年第3期136-139,共4页
Chinese Journal of Prosthodontics