He-Ne激光对再生肝细胞影响的扫描电镜研究

SEM OF REGENERATING HEPATOCYTES AFFECTED BY He-Ne LASER

本文首次应用扫描电镜探讨了He-Ne激光照射对大鼠肝部分切除后再生肝细胞的影响。再生肝细胞通过剃毛肝区隔胶接受激光照射。受照组肝细胞其再生早期的应急变化明显较对照组轻,但其胞质内脂滴含量显著多于对照组,且可观察到旺盛的类顶浆分泌。术后72小时,受照组肝细胞的胆小管比对照组增生显著,可见多个分枝。术后7天,受照组肝细胞的内部结构和外表形态均优先于对照组恢复于正常状态,旦其微绒毛的生长超过正常肝细胞。实验结果表明,He-Ne激光照射对再生肝细胞的再生过程具有显著促进作用。

The effects of He-Ne Laser on the rat liver regeneration after partial hepatectomy is studied by SEM. The regenerating liver was irradiated through the shaved abdoman wall. Comparing with non-irradiated group, the urgent reaction responsive to partial hepatectomy is much reduced in irradiated group and the lipid droplet is much increased within its cells. The appearance of actve apocrine secretion in irradiated regenerating hepatocytes is observed, but no appearance in non-irradiated group. This phenonenon has not deen found in liver cells before. It is obvious that the bile canaliculus in irradiated group are enhanced to proliferate and its inner and surface structures gain a earlier restoration in morphology than in non irradiated group. The conclusion comes to that He-Ne Laser can help to promot the regenerating process of hepatocytes.