野生与栽培黄芩中总黄酮及黄芩苷含量的比较研究

Comparative Study on Total Flavonoids and Baicalin Content between Cultivated and Wild Radix Scutellariae

目的:比较野生黄芩与栽培黄芩中总黄酮及黄芩苷的含量。方法:采用紫外分光光度法测定黄芩中总黄酮的含量,采用高效液相色谱法测定黄芩苷的含量,比较野生黄芩与人工栽培黄芩中总黄酮及黄芩苷含量的差异。结果:野生黄芩总黄酮的含量为16.31±2.91%,栽培黄芩总黄酮的含量为25.60±3.03%,存在显著性差异(P=0.005);野生黄芩黄芩苷的含量为8.46±1.23%,栽培黄芩黄芩苷的含量为11.66±1.36%,存在显著性差异(P=0.016)。栽培黄芩总黄酮和黄芩苷的含量显著高于野生黄芩。结论:从总黄酮和黄芩苷含量角度而言,栽培黄芩优于野生黄芩,可以替代野生黄芩用于临床和实际应用。

Objective： To do some comparative study on the content between the wild and cultivated samples of Radix Scutellariae . Mcthods： of total flavonoids and baicalin Ultraviolet spectrophotometry was used to determine the total flavonoids content and baicalin was determined by HPLC. Results： Total flavonoids content of wild Radix Scutellariae reached 16.31 ±2. 91%,and that of cultivated samples reached 25.60±3.03%, the both have significant difference （P=0.016）.The baicalin content of wild and cultivated Radix Scutellariae were 8. 46±1.23% and 11.66±1.36%respectively in the two groups with notable difference （P=0.016）.The content of total flavonoids and baicalin of cultivated Radix Scutellariae were obviously higher than those of wild Radix Scutellariae Conclusion： Cultivated Radix Scutellariae was better than wild one from the perspectives of total flavonoids and baicalin content and could replace wild Radix Scutellartiae in clinical study and application.