甲基丙烯酸酯类毛细管整体柱分离极性物质的研究

Study on separation of polar compounds using polymethacrylate based capillary monolithic column

以甲基丙烯酸缩水甘油酯、甲基丙烯酸、2-二甲基乙基胺甲基丙烯酸酯为单体,乙二醇二甲基丙烯酸酯为交联剂,偶氮二异丁腈为引发剂,十二醇和环己醇为二元致孔剂,原位聚合制备了聚(甲基丙烯酸缩水甘油酯-乙二醇二甲基丙烯酸酯-2-二甲基乙基胺甲基丙烯酸酯-甲基丙烯酸)毛细管液相色谱整体柱.然后在酸性条件下,对甲基丙烯酸缩水甘油酯的环氧环进行开环反应生成二醇,增加了固定相表面的极性.通过简单的改变流动相乙腈浓度,分析过程表现为不同的作用机理.在高乙腈浓度下为典型的亲水作用机理.对于带电物质,固定相与分析物间的静电作用也对保留起到一定作用,为极性组分分离提供更多的选择条件.

A capillary monolith column was prepared for capillary high performance liquid chromatography （μ-HPLC） by in-situ thermal polymerization of glycidyl methacrylate, methacrylic acid, 2-（dimethyl amino） ethyl methacrylate and ethylene dimethacrylate, and then hydrolyzed with hydrochloric acid, the epoxy groups on the surface were hydrolyzed to diol groups to enhance the polarity of the stationary phase. By simply altering the ACN content in the mobile phase, two mixed-mode mechanisms could be achieved on the same monolithic column. Hydrophilic interaction is observed at high ACN content, as well as hydrophobic interaction is observed at low ACN content.