Tb^(3+)与氧合血红蛋白反应动力学的停流荧光光度法研究

Studies on Kinetics of Reaction Between Tb 3+ and Human Oxyhemoglobin by Stopped flow Fluorometry

近年来，稀土在农业中的应用引起人们对它的安全性的关注．Ｅｖａｎｓ［１］曾报道稀土化合物由于其类Ｃａ２＋性导致各种负面生物效应，包括影响心血管功能及溶血．然而有关稀土离子与血红蛋白的研究工作国内外尚很少见报道．我们发现稀土离子能影响血红蛋白的载氧功能［...

The kinetics of reaction between Tb 3+ and human oxyhemoglobin was investigated by means of stopped flow fluorometry. The Tb 3+ ions quenched the intrinsic fluorescence of oxyhemoglobin within the first 100 ms. The rate constant of the reaction at various temperature and activation parameters were obtained. The results indicate that Tb 3+ binding to oxyhemoglobin is attributed to the second order reaction. The mechanism of the reaction was explored.