海南兰花病毒病调查及病原检测

A Survey to and Pathogen Identification of Virus Diseases of Orchid in Hainan

2007～2008年,对海南主要兰花种植基地进行病毒病危害情况调查。结果表明,海南兰花病毒病症状复杂,以斑驳花叶型和坏死型最为普遍。调查还发现,兰花的病毒病发病率与龄期呈正相关,文心兰、石斛兰的发病率显著高于蝴蝶兰。病原血清学检测表明,海南兰花病原病毒主要为建兰花叶病毒、齿兰环斑病毒,以及二者复合感染,检出率分别为29.5%、2.3%、15.9%。其中文心兰和蝴蝶兰带毒率较高,石斛兰带毒率最低,石斛兰上未检出齿兰环斑病毒。

Virus diseases of orchids in major orchid gardens were investigated in Hainan Province during 2007 to 2008. The investigation showed that symptoms of virus diseases in the orchid were considerably complicated, with the mild mottle and the necrosis being the most common in orchid. The investigation also showed that the virus infection rate was positively related to the age of orchids, and that the infection rates were obviously higher in Oncidium and Dendrobium than in Phalaenopsis. The double antibody sandwich enzyme-linked immunosorbent assay （DAS-ELISA） revealed that Cymbidium mosaic virus （CymMV）, Odontolossum ring spot virus （ORSV） and their mixed infections were more common, with the infection rates being 29.5%, 2.3% and 15.9%, respectively. Oncidium and Phalaenopsis were more sensitive to viruses than Dendrobium, and ORSV was not found in the Dendrobium orchid.