益气活血中药对放射性肺损伤大鼠血清及肺组织ET-1表达影响的实验研究

Experimental Study of Effect of Yiqi Huoxue Recipe on the Serum and Pulmonary ET-1 Expression in the Iung Injury Induced by Thoracic Radiation of Rats

目的探讨血浆及肺组织中ET-1在放射性肺损伤中的改变及益气活血中药对其治疗作用。方法96只Wistar种雌性大鼠随机分为照射加中药组(A组)30只、照射加氨溴索组(B组)30只、单纯照射组(C组)30只、正常对照组(D组)6只。用直线加速器对前三组大鼠右肺进行分次照射(5Gy/次,1次/周,累积剂量为30Gy),于照射开始后第4、6、8周3个时间点分别处死三组大鼠各6只,采血测定ET-1浓度,肺组织测定ET-1免疫组化。结果照射后肺组织炎症和肺水肿进行性加重;C组照射后5,15,30,60d血清及肺组织ET-1水平进行性升高,A组、B组各时相值表达相似,均明显低于C组,差异具有统计学意义(P<0.05)。结论血浆ET-1水平的变化能反映放射性肺损伤的严重程度,益气活血中药能抑制肺组织ET-1的表达,降低血浆ET-1水平,可能是益气活血中药治疗放射性肺损伤的一个作用机制。

Objective To explore the changes of endothelin-1 in plasma and lung tissues, and the curative effect of Yiqi Huoxue Recipe （Chinese traditional medicine of nourishing qi and activating blood） in radiation-induced lung injury. Methods 96 wistar rats were randomly divided into irradiation group, irradiation with Yiqi Huoxue Recipe - treated group, irradiation with ambroxol- treated group and normal control group. The first three groups were irradiated at right hemithorax by linear accelerator with a dose of 30 Gy 6 Fraction 5 weeks. Animals were followed up for 26 weeks after the first irradiation. The contents of ET-1 in plasma and lung tissues were measured by ELISA and immunoreactivity repectively at the end of the 4th, 6th, 8th, 12th, and 26th week after the first irradiation. Results Pulmonary edema and pneumonia aggravated gradually in C group, pulmonary edema and pneumonia of A and B group were lighter than C group, the ET-1 levels in plasma and lung tissues increased rapidly in C group, the ET-1 levels in plasma and lung tissues of A group were similar to that of B group （P 〉0.05）, and were both lower than C group （ P 〈0.05）. Conclusion ET-1 could reflect the degree of radiation lung injury. Yiqi Huoxue Recipe could degrade the levels of ET-1 in plasma and lung tissues significantly. This might be one of their mechanisms in treatment of radiation-induced lung injury.