HPLC法测定马来酸氯苯那敏中间体CPA的含量

Quantitative Determination of CPA by HPLC

建立HPLC方法测定马来酸氯苯那敏中间体CPA（以下简称CPA）的含量。采用迪马Diamonsil（TM）C18（5μm×4．6mm×250mm）色谱柱；w（乙腈）：w（甲醇）：w（四氢呋喃）：w（水）=（27：8：5：60）（pH=3．5）为流动相，流速为1．2mL／min，检测波长为254nm，进样体积为20μL。结果显示：CPA的进样量在0—10．12μg范围内与主峰面积呈良好的线性关系，r=0．9999。平均回收率为99．4％，RSD为0．23％（n=9）。该方法简便、灵敏、准确度高，适用于CPA的含量测定。

HPLC methods for the determination of CPA was established. Diamonsil（TM）C18 （5 μm ×4.6 mm×200 mm） was used, and the mobile phase was composed of acetonitrile - methanol - tetrahydrofuran - water （ pH 3.5 ） （27： 8：5： 60）. The detection wavelength was at 254 nm with the flow rate of 1.2 mL/min. The results show the linear range is 0 - 10. 12 μg for CPA. r = 0.999 9. The average recoveries of CPA are 99.4% , RSD = 0.23% （n = 9）. This method is rapid, simple and accurate.