氨苄青霉素和莫西沙星对流感嗜血杆菌生物被膜的抑制作用

Inhibition of ampicillin and moxifloxacin on biofilms produced by haemophilus influenzae

目的:探讨氨苄青霉素和莫西沙星对体外流感嗜血杆菌早期及成熟生物被膜的抑制作用。方法:通过结晶紫染色测定吸光度值的变化,观察不同浓度下两种抗生素对早期及成熟生物被膜的影响。结果:1/8、1/64MIC浓度的氨苄青霉素和莫西沙星可抑制流感嗜血杆菌的粘附以及生物被膜早期的形成,1/8、1、8MIC浓度的莫西沙星可破坏成熟的流感嗜血杆菌生物被膜。结论:低浓度氨苄青霉素和莫西沙星可抑制流感嗜血杆菌生物被膜的形成,莫西沙星可破坏成熟的生物被膜。

Objective： To evaluate the inhibition of ampicillin and moxifloxacin on initial and mature biofilms formed by Haemophflus influenzae in vitro. Methods ： The effects of initial and mature biofilms were measured by crystal violet assay under various concentrations of ampicillin and moxifloxacin. Results： At concentrations of 1/8 and 1/64 MICs, bacterial adhesion and initial biofilm synthesis were inhibited by ampicillin and moxifloxacin. Disruption of mature biofilms could be achieved by moxi- floxacin at concentration of I/8, 1, 8MIC. Conclusion： Haemophilus influenzae biofilms might be inhibited at lower concentrations of ampicillin and moxifloxacin in vitro. Moxifloxacin can break down mature biofilm.