腹腔巨噬细胞对急性胰腺炎大鼠腹腔炎症反应的影响

Effect of peritoneal macrophages on peritonitis of rats with acute pancreatitis

目的:研究腹腔巨噬细胞(peritoneal macrophages,PMs)对实验性胰腺炎大鼠腹腔炎症反应的影响。方法:胰胆管逆行注射2%去氧胆酸钠制作出血坏死性胰腺炎大鼠模型,使用氯屈磷酸二钠脂质体(Liposome-CL2MBP)腹腔注射特异性清除腹腔巨噬细胞,采用纯雄性S-D大鼠24只,随机分为腹腔巨噬细胞保留/PMs(+)组、腹腔巨噬细胞清除/PMs(-)组和假手术/SO组。造模后12h活杀大鼠取标本,做胰腺病理学检查,血清淀粉酶测定,腹水定量,检测腹水细胞因子和一氧化氮水平。结果:PMs(-)组和PMs(+)组间在胰腺病理学评分、血清淀粉酶水平方面无显著差异,两组在腹水定量、腹水细胞因子和一氧化氮水平方面差异显著(P<0.05)。结论:腹腔巨噬细胞对急性胰腺炎大鼠腹腔炎症反应有重要影响。

Objective： To study the effect of peritoneal macrophages （ PM ） on peritonitis of rats with experimental pancreatitis. Methods： A model of hemorrhagic necrotic pancreatitis was established by retrograde injection of 2% sodium deoxycholate into the common biliopancreatic duct of rats. Liposome-CL2MBP was injected into the peritoneum to exhaust PM of rats. Twentyfour male rats were randomly divided into PMs（ ＋ ） group, PMs（-） group and sham operation group. The rats were killed 12h after the model was established to take samples. Pancreatic pathology was examined. Ascite lavage was quantified. Serum AMY, eytokine and NO levels were measured. Results： No significant difference was found in pancreatic pathology and serum AMY level between PMs（ ＋ ） and PMs（-） groups, but a significant difference was observed in aseite lavage quantity and serum cytokine and NO level between the two groups. Conclusion： PM have important effects on peritonitis of rats with acute pancreatitis.