偶合标记铜离子催化放大的极谱免疫分析法测定破伤风类毒素

Determination of Tetanus Toxoid by Polarographic Immunoassay Coupled With Catalytic Amplification of Labeled Copper Ion

建立了测定破伤风类毒素的铜离子标记极谱免疫分析方法．用二乙三胺五乙酸（ＤＴＰＡ）酸酐法将用离子标记于抗原破伤风类毒素（ＴＴ）．所得标记物中ＴＴ：ＤＴＰＡ：Ｃｕ的摩尔标记比为 １：６：６、铜离子催化底ｏ－苯二胺（ＯＰＤ）的氧化反应，生成电活性产物 ２，３－二氧基吩嗪（ＤＡＰ），以单扫描极谱法检测ＤＡＰ．采用异相竞争免疫分析模式，本方法可测定０．０５～１ｍｇ／Ｌ ＴＴ。

A method for the determination of tetanus toxcoid (TT) by using polarographic immunoassay coupled with catalytic amplification of the labeled copper ion was described. In this method, copper ion was labeled to antigen tetanus toxoid (TT) through diethylenetriamine pentaacetic acid (DTPA) anhydride. The molar labeled ratio of TT: DTPA: Cu in the conjugate wag identified to be l: 6: 6. The labeled copper ion as catalyst catalyzed the oxidation of substrate o-phenylenediamine(OPD) to generate an electroactive product 2, 3-di aminophenazine(DAP), the product DAP was then detected with a linear-sweep polarogra phy. The heterogeneous competitive format was employed, the proposed assay can determine 0. 05-l mg/L of TT.