污水处理工艺对氨氧化菌及细菌群落的影响

Impacts of wastewater treatment process on AOB and bacteria communities

采用针对氨氧化菌(AOB)功能基因氨单加氧酶(amoA)的末端限制性片段长度多态性技术(T-RFLP)、克隆测序等方法,研究了北京市2个污水处理厂的4个污水处理系统中AOB的群落结构,同时采用针对16SrRNA基因的T-RFLP技术分析了总细菌的群落结构.T-RFLP指纹图谱分析表明,4个污水处理系统中AOB的优势限制性片段(T-RF)均为291bp和354bp,细菌的优势T-RF为115,117,166,455,465,468,471,482,800,893bp等.说明污水处理工艺对系统中AOB及细菌的群落结构影响很小.对功能基因amoA的系统发育分析表明,4个污水处理系统中优势AOB均属于Nitrosomonas europaea cluster和Nitrosomonas oligotropha culster.

Communities of AOB in activated sludges collected from 4 wastewater treatment systems in 2 plants in Beijing were examined using specific PCR followed by terminal restriction fragment length polymorphism （T-RFLP）, cloning, and sequencing of amoA genes. Communities of bacteria were also investigated by using T-RFLP based 16S rRNA genes. The T-RFLP fingerprints analysis showed that the dominant terminal restriction fragments （T-RFs） of AOB were 291,354 bp, and the dominant T-RFs of bacteria were 115, 117, 166, 455, 465, 468, 471,482, 800 and 893 bp etc. in all these 4 systems. The T-RFLP profiles analysis may also indicate the AOB and bacteria structures were slightly affected by the treatment process. Phylogenetic analysis of cloned amoA genes clearly showed that the dominant AOB in these 4 systems were members ofNitrosomonas europaea cluster and Nitrosomonas oligotropha cluster.