混合培养条件下几种赤潮藻对无机磷源的竞争生长研究

Research on competiting growth of some harmful algae to phosphate in co-exist bath incubation

采用批次培养方式,研究混合培养的几种赤潮藻东海原甲藻(Prorocentrum donghaiense)与中肋骨条藻(Skeletonema costatum)及东海原甲藻与链状亚历山大藻(Alexandrium catenella)对溶解无机磷源的竞争生长响应。结果表明,在富磷及贫磷的培养条件下,中肋骨条藻的比生长率远超过东海原甲藻,而成为培养体系中的绝对优势种。在东海原甲藻与链状亚历山大藻的混合培养体系中,原甲藻大量死亡,可能存在亚历山大藻对原甲藻的他感作用。培养体系中,碱性磷酸酶活性随藻类磷胁迫而显著升高,可能在后期种群利用代谢有机磷源时发挥重要作用,且在不同培养体系中表现出酶活性大小及状态的差异性,该结果可能影响浮游植物对有机磷源的利用效率。

The co-exist batch incubation experiment of harmful algae Prororenctrurn donghaiense ＆ Skeletonerna costaturn （PS） and P. donghaiense ＆ Alexandriurn catenella were carried out to study the competiting growth response of these species to phosphate. The results showed that the growth rate of S. costaturn was far higher than that of P. donghaiense whatever in the P-rich or in the P-scarce condition and became the absolutely dominant species. While in the co-exist culture of P. donghaiense and A. catenella, P. donghaiense went death and this might be due to the allelopathy of A. catenella. Alkaline phosphatase activity （APA） increased greatly with P stress and might play an important role in controlling population competition via hydrolyzing metabolized dissolved organic phosphorus in the latter period.