26种中草药对奶牛隐性乳房炎病原菌体外抗菌作用的研究

Antibacterial effects of 26 Chinese herbal medicines on pathogens of dairy cow with subclinical mastitis in vitro

研究26味中草药对隐性乳房炎分离得金黄色葡萄球菌和大肠杆菌的体外抗菌效果。杯碟法试验表明,黄连、黄芩、紫花地丁、板蓝根等对2种病原菌的综合抑菌效果较好,达到中敏或高敏,有的甚至是极敏,抑菌圈直径为19.00～24.93 mm。微量稀释法试验表明,黄连、黄芩、红花、金银花、板蓝根等对2种病原菌的综合效果好,MIC为0.004～0.063 g/mL,MBC为0.004～0.063 g/mL。本试验可为研究外用治疗奶牛乳房炎中草药制剂的开发提供科学依据。

The in vitro antibiotic tests of 26 Chinese herbal medicines, such as Rhizoma coptidis, Radix scutellariae, Herba taraxaci and Herba violae, etc. were conducted on the isolated Staphylococcus aureus and Escherichia coli from the dairy cow with subclinical mastitis by cylinder plate and microdilution methods. The result of the plate method test indicated that the Rhizoma coptidis, Radix scutellariae, Herba violae, Radix isatidis etc. were more effective compared with other Chinese herbat medicines on the S. aureus and E. coli, and their sensitivity to the 2 pathogens are moderate or highly, or extremely high, the range of antibacterial diameters was 19. 00- 24.93 mm. The result of the microdilution method test also showed that the Rhizoma coptidis, Radix scutellariae, Flos carthami, Flos Ionicerae, Radix isatidis, etc. were more effective compared with other Chinese herbal medicines on the S. aureus and E. coil, their MIC was 0. 004 -0. 063 g/mL, and their MBC was 0. 004 - 0. 063 g/mL respectively. This study provides a scientific basis for the development of traditional Chinese medicines on the external treatments of the mastitis in dairy cow.