黄芪皂苷和黄芪多糖对乳鼠肥大心肌细胞线粒体膜电位的影响

Influence of astragalus saponin or astragalan polysaccharide on MMP of hypertrophic cardiomyocyte in neonatal rats

目的观察黄芪皂苷和黄芪多糖对心肌细胞线粒体膜电位(mitochondrial membrane potential,MMP)的干预作用,初步探讨黄芪防治心衰的机理。方法将体外培养乳鼠心肌细胞分为对照组、模型组、黄芪皂苷组、黄芪多糖组、黄芪皂苷+多糖组。除对照组外各组加血管紧张素Ⅱ(AngⅡ)造成肥大细胞模型,给药组予黄芪皂苷、黄芪多糖干预,分别测定各组24h、48h时心肌细胞MMP。结果24h时MMP比较:模型组较对照组下降,有显著性差异(P<0.01);3个给药组较模型组升高,有显著性差异(P<0.01);黄芪皂苷组较对照组升高,有显著差异(P<0.01)。48h时MMP比较:模型组较对照组下降明显(P<0.01);3个给药组较模型组升高,以黄芪皂苷组、黄芪皂苷+多糖组明显,与模型组比较有显著性差异(P<0.01)。结论AngⅡ使心肌细胞MMP下降,黄芪皂苷和黄芪多糖使MMP趋于正常,黄芪皂苷和黄芪多糖可拮抗AngⅡ减低心肌细胞MMP的作用,这可能是黄芪防治心衰的机理之一。

Objective To observe the intervention effect of astragalus saponin or astragalan polysaccharide on mitochondrial membrane potential （MMP） of cardiomyocyte, and investigate initially the mechanism of preventing and treating heart failure by Radix Astragali seu Hedysari. Method The cardiomyocytes were cultured in vitro in neonatal rats, and then divided into the control group （group 1）, model group （group 2）, astragalus saponin model （group 3）, astragalan polysaccharide group （group 4） and astragalus saponin combined with astragalan polysaccharide group （group 5）. All groups, except group 1, were given angiotensin Ⅱ （AngⅡ） for establishing the model of hypertrophic cardiomyocyte. Group 3, 4 and 5 were intervened by astragalus saponin or astragalan polysaccharide respectively. The MMP of cardiomyocyte was detected in all groups on 24th and 48th hour. Result On 24th hour, MMP decreased in group 2 than that in group 1 with a significant difference （P〈0.01）, increased in group 3, 4 and 5 with a significant difference （P〈0.01）, and increased in group 3 than that in group 1 with a significant difference （P〈0.01）. On 48 hour, MMP decreased more significantly in group 2 than that in group Ⅰ （P〈0.01）, increased in group 3, 4 and 5, and more significantly in group 3 and 5 than that in group 2 with a significant difference （P〈0.01）. Conclusion AngIl can reduce MMP in cardiomyocytes. Astragalus saponin and astragalan polysaccharide can promote MMP to the normal level, which indicates that astragalus saponin and astragalan polysaccharide can antagonize the MMP decreasing effect of AngⅡ. It may be one of mechanisms of Radix Astragali seu Hedysari for preventing and treating heart failure.