利用啤酒废水小球藻异养培养

Heterotrophic Cultivation of Chlorella for Beer brewery wastewater treatment

【目的】利用小球藻异养培养技术处理啤酒废水,旨在为啤酒废水资源化利用和降低小球藻生产成本提供一条途径。【方法】在含有10g/L葡萄糖的基本培养基进行异养小球藻高效藻株的筛选,并用于啤酒废水的资源化处理。【结果】从5株小球藻中得到2株适合高密度异养培养的藻株(Chlorella pyrenoidosa 15-2070和Chlorella vulgaris 15-2075),在啤酒废水的资源化处理过程中这2株小球藻得到非常接近的实验结果。利用由废水配制含10g/L葡萄糖的基本培养液培养Chlorella pyrenoidosa 15-2070获得了5.3g/L藻细胞;并且在此过程中,啤酒废水得到有效利用,几种主要污染物最高去除率为:CODcr,92.2%;BOD5,95.1%;NO3--N,98.5%;NH4+-N,92.3%。【结论】啤酒废水中的重要环境污染物在培养小球藻的过程中可以得到有效地清除,并从中可以获得具有商业价值的小球藻细胞。

[Objective] To treat wastewater from beer breweries, we used the wastewater for the heterotrophic cultivation of Chlorella. [ Methods] Using Basal medium containing 10 g/L glucose·H2O, we screened 5 Chlorella strains, of which 2 strains with high specific growth rate and maximal biomass were used for wastewater treatment. [ Results] The two strains, Chlorella pyrenoidosa 15 - 2070 and Chlorella vulgaris 15 - 2075, were suitable for heterotrophic mass cultivation, and had similar results. For Chlorella pyrenoidosa 15 - 2070, 5.3 g/L biomass was achieved with Basal medium containing 10 g/L glucose· H2O and beer wastewater. In addition, the highest removal efficiencies for CODcr （92.2%）, BOD5 （95.1% ）, NO3^- -N （98.5%）, NH4^＋ -N （92.3 % ） were achieved during the process. [ Conclusion] Beer wastewater could be used as a major component of the medium for heterotrophic cultivation. Most of the harmful compounds in the wastewater could be removed during this process.