辣椒抗白粉病人工接种鉴定方法研究

Studies on Artificial Inoculation Method to Test Resistance to Pepper Leveillula taurica

以不同抗感病辣椒品种(系)为试材,从接种浓度、调查时间、接种植株苗龄、接种后发病条件、接种植株处理方式等方面对辣椒抗白粉病人工接种鉴定方法进行了研究。结果表明,辣椒抗白粉病的最佳人工接种鉴定方法为:用含孢子1×104个.mL-1的菌悬液接种,接种15d后进行抗病性鉴定,15片真叶期为接种鉴定苗龄,接种后黑暗保湿24h,随后转为白天温度23～27℃,相对湿度60%～85%,夜间温度16～18℃,相对湿度95%～100%的环境条件适宜白粉病菌的侵染。

Taking different disease resistance pepper varieties （lines） as material, this paper studies on the artificial inoculation as identification method of Leveillula taurica to pepper from inoculation concen- tration, investigation time, age of inoculated seedling, disease occurring condition after inoculation plant inoculation treatment method, etc. The results showed that the most effective artificial inoculation method was inoculating at 15-leaf stage and sprayed by a suspension of 1 × 10^4 conidia mL-1 and kept in dark and under 100 % humidity during first 24 h after inoculation and then in 23 -27 ℃ and 60 % -85 % humidity in daytime, 16 - 18 ℃ and 95 % - 100 % humidity at night.