胃癌及其癌前病变相关新基因GP1的克隆

Cloning of novel gene GP1 associated with gastric cancer and its premalignant lesions

目的比较胃癌、癌前病变和正常胃黏膜之间基因表达的差异,寻找与胃癌发生、发展相关的基因。方法用荧光mRNA差异显示技术(FDD)分离差异表达的基因片段,进行PCR再扩增。将扩增的cDNA片段克隆后进行测序,测序结果提交GenBank,经BLAST检索进行同源性分析。差异条带经Northern杂交验证。应用SMART-RACE技术扩增GP1的全长cDNA,并应用生物信息学技术预测该基因的生物学功能。结果发现1个在胃癌及癌前病变组织中低表达的而且在GenBank数据库中未找到同源序列的cDNA片段,为新的基因片段,GenBank号CD454989。GP1的全长cDNA序列为1362bp,编码生物学功能未明的具有267氨基酸的蛋白质BAA91562.1。结论发现了一个在胃癌、癌前病变及正常胃黏膜组织中差异表达的新基因,它可能与胃癌相关。

Objective To explore the and premalignant lesions. Methods differentially expressed genes of gastric cancer, matched normal gastric tissue The differentially expressed cDNA bands were isolated and identified by fluorescent differential display and then reamplified by PCR. After being cloned, all cDNA fragments were sequenced. Through BLAST, the results were compared with GenBank database for homology analysis. The expression of the cDNA fragment in different tissues was confirmed by Northern blot. SMART-RACE （rapid amplication of cDNA ends） was used to amplify the full length cDNA sequence. Bioinformatics analysis was performed to analysis its function. Results A differentially expressed cDNA fragment expressed lower in gastric cancers and premalignant lesions, no homology was found in GenBank. The novel cDNA fragment was given an accession number of EST （CD454989）in GenBank. A full length cDNA sequence of 1362 bp was acquired, who coded 267 amino acids, and was homologous with BAA91562. 1, whose physiology function was unknown. Conclusion A differentially expressed gene was found and it may be involved in process of the gastric cancer.