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昆虫细胞BTI-Tn-5B1-4和Sf-9的无血清培养及其特性研究 被引量:1

Study on Characteristics of BTI-Tn-5B1-4 and Sf-9 Cells Adapted in Serum-free Medium
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摘要 本研究对目前应用最多的两种昆虫细胞BTI-Tn-5B1-4和Sf-9在无血清培养基Sf-900Ⅲ中进行驯化和培养,现已稳定传至45代以上。比较了两种细胞在Sf-900Ⅲ和有血清培养基TNM-FH中的细胞形态、生长速率、病毒产量和重组蛋白表达水平。结果表明,在Sf-900Ⅲ中Sf-9细胞比TNM-FH培养的略大,BTI-Tn-5B1-4的圆形细胞增多,BTI-Tn-5B1-4和Sf-9在Sf-900Ⅲ中生长速度快,群体倍增时间分别为18.5h和21.7h,而在TNM-FH中,BTI-Tn5B1-4和Sf-9的群体倍增时间分别为21.9h和25.4h;在两种培养基中BTI-Tn-5B1-4和Sf-9细胞的病毒感染率、多角体产量和β-半乳糖苷酶(β-galactosidase)的表达水平均无明显差异,而碱性磷酸酶(secreted alkaline phosphatase,SEAP)在Sf-900Ⅲ中的表达水平明显高于TNM-FH中的水平。 BTI -Tn -5B1 -4 and Sf- 9 cell lines, that are dominant cell lines with basic study and application in the world, were adapted to a serum -free medium, Sf- 900 m for approximately 45 passages. The characteristics including morphology, growth kinetics, virus production and recombinant protein expression were compared with that of they grew in Sf- 900 m and TNM -FH. The results showed that the two cells line in Sf- 900Ⅲ were little bigger than in TNM -FH. The cell population doubling times of both BTI -TnSB1 -4 and Sf- 9 cells in Sf- 900Ⅲ(18.5 and 21.7 h, respectively) shortened in comprision to that in TNM -FH(21.9 h for BTI- TnSB1 -4 and 25.4 h for Sf - 9). The virus infection rate, production of polyhedra and expression of β - galactosidase (β - gal) in both types of media were not significantly different, but the expression of secreted alkaline phosphatase (SEAP) in Sf- 900Ⅲ medium was higher than in serum -containing cultures.
出处 《青岛农业大学学报(自然科学版)》 2009年第2期131-135,共5页 Journal of Qingdao Agricultural University(Natural Science)
基金 国家自然科学基金(30771451) 国家973计划(2009CB118902)
关键词 昆虫细胞 无血清培养 生长曲线 病毒产量 蛋白表达 cell lines serum - free culture growth kinetics virus production protein expression
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