摘要
以小鹅瘟病毒(GPV)VP1-VP3非重叠序列重组原核表达多肽为检测抗原,建立了鉴别GPV全病毒抗体与VP3亚单位抗体的Dot-ELISA。该方法中检测抗原包被浓度为70ng/斑点,兔抗鹅IgG-HRP标记抗体的最适稀释度为1∶200,被检血清最佳稀释度为1∶400。特异性试验的结果中,检测抗GPV抗体的阳性率为100%,而抗GPV-VP3禽痘重组病毒抗体的阳性率为0,表明其有很好的特异性与灵敏度。
To identificatie goose parvovirus （GPV） antibody and VP3 subunit antibody,Dot-ELISA was established with recombinant prokaryotic expressed peptide of GPV-（VP1-VP3）non-repeated nucleotide sequences. Coating density was 70 ng/dot in the method. The optimum dilution of rabbit anti-goose lgG-HRP conjugated antibody and detected serum were 1：200 and 1：400,respectively. Specificity assay showed that positive rates of GPV antibody and serum antibody agaist GPV-VP3 gene recombinant poultry poxvirus were 100% and 0,respectively. It was showed that this method had high specificity and sensitivity.
出处
《中国家禽》
北大核心
2009年第11期24-26,共3页
China Poultry
基金
内蒙古民族大学博士科研启动基金资助
