活性艳蓝X-BR降解菌的筛选

Screening of Bacteria Degrading Reactive Brilliant Blue X-BR

从某印染厂废水排放出口的污泥中分离到一株活性艳蓝X—BR染料高效降解菌LPY68—14，经生理生化鉴定，该菌为埃希氏菌（Escherichia sp．）。研究了影响菌株LPY68—14降解效果的因素，实验结果表明：在缺氧、质量分数为0．2％葡萄糖为外加碳源、温度37℃、接种量4mL、pH7的最佳条件下，质量浓度为30mg／L的活性艳蓝X—BR经菌株LPY68—14处理24h后的降解率可达80％；当活性艳蓝X—BR质量浓度为100—400mg／L时，该菌处理48h后的降解率可稳定在70％左右。

A strain of bacterium which can effectively degrade reactive brilliant blue X-BR, LPY68-14 was isolated from the sludge in the wastewater discharge port of a dying plant. According to its physiological and biochemical characteristics, the strain LPY68-14 is Escherichia sp.. The factors affecting the degradation were studied. The experimental results show that： Under the optimum conditions of anoxic environment, using glucose with 0. 2% of mass fraction as external carbon source, temperature 37 ℃, inoculum dosage 4 mL, and pH 7, the degradation rate of reactive brilliant blue X-BR with 30 mg/L of mass concentration can come up to 80% after 24 h of treatment by LPY68-14; When the mass concentration of reactive brilliant blue X-BR is 100 -400 mg/L, its degradation rate can be kept at about 70% after 48 h of treatment.