林蛙油复方冲剂对超重环境下微波辐射大鼠肝脏TGF-β_2 mRNA表达的影响

Effect of Rana Japonica Oil Compound Granules on the Expression of TGF-β_2 mRNA in the Liver of the Microwave Radiation Rats under Hypergravity Environment.

目的观察林蛙油复方冲剂对超重环境下微波辐射大鼠肝脏TGF-β_2mRNA表达的影响。方法Wistar大鼠复制超重环境和微波辐射协同作用动物模型,林蛙油复方冲剂进行干预14d后,采用RT-PCR法检测各组大鼠肝脏TGF-β_2mRNA的表达。结果与空白对照组比较,超重辐射实验组和复方冲剂防护后超重辐射组TGF -β_2mRNA表达显著升高(P<0.05);复方冲剂实验组和复方冲剂防护前超重辐射组TGF-β_2mRNA表达显著降低(P<0.05);复方冲剂防护中超重辐射组TGF-β_2mRNA表达略有降低,但无统计学意义(P>0.05)。结论超重环境和微波辐射协同作用可增强大鼠肝脏TGF-β_2mRNA表达,林蛙油复方冲剂可降低正常大鼠和超重环境下微波辐射大鼠肝脏TGF-β_2 mRNA表达。

Objective To study the effect of Rana Japonica Oil Compound Granules on the expression of TGF - β2 mRNA in the liver of the microwave radiation rats under hypergravity environment. Methods The models of Wistar rats under synergistic action of hypergravity environment and microwave radiation were established and intervened 14 days with Rana Japonica Oil Compound Granules to examines the expression of TGF - β2 mRNA in the liver from each group of rats by RT - PCR. Results Compared with blank control group, the expression levels of TGF - β2 mRNA in the liver increased significantly in hypergravity radiation experimental group and hypergravity radiation after compound granules protection group （ P 〈 0.05 ） and significantly;decreased in compound granules experimental group and hypergravity radiation before compound granules protection group （ P 〈 0.05 ） and slightly decreased in hypergravity radiation In compound granules protection group, but no statistical significance （ P 〉 0. 05 ）. Conclusion Synergistic action of hypergravity environment and microwave radiation may strengthen the expression of TGF - β2 mRNA in the liver of rats ; Rana Japonica Oil Compound Granules may reduce the expression of TGF - β2 mRNA in the liver of the normal rats and the microwave radiation rats under hypergravity environment.