半枝莲多糖B3-PS2分离纯化及抗补体活性研究

Anti-complement activity of polysaccharide B3-PS2 purified from Herba Scutellariae Barbatae

本文提取半枝莲多糖,分别经DEAE-cellulose、Sephacryl S-300柱层析分离纯化后得到均一的B3-PS2组分,并对该组分进行了抗补体活性研究。研究结果表明B3-PS2组分分子质量为1100kD,主要由Glc、Gal和Ara三种单糖组成,糖残基摩尔比为2.7∶2.7∶1.0,还含有少量的Man、Rha、Fuc和Xyl。B3-PS2抑制补体活性的经典途径CH50为(0.23±0.03)mg·mL-1,抗补体作用靶点为补体C1r、C1s、C3、C4组分。半枝莲多糖B3-PS2组分体外抗补体活性接近于阳性对照肝素,表明活性较好,这显示了半枝莲多糖治疗补体过度激活相关疾病的潜在价值。

The polysaccharide B3-PS2 was extracted and purified from Herba Scutellariae Barbatae through chromatography of DEAE-cellulose and Sephacryl S-300 column. Average molecular weight of B3-PS2 was about 1 100 kD. It was composed of Glc, Gal and Ara in the ratio of 2.7 ： 2.7 ： 1.0, along with trace of Man, Rha, Fuc and Xyl. B3-PS2 inhibited complement activation on the classic pathways with CH50 value of （0.23±0.03） mg·mL^-1. The targets of B3-PS2 upon the complement system were C1r, C1s, C3 and C4. These results suggested that anti-complementary activity of B3-PS2 was closed to its positive control heparin. It strongly suggested that the polysaccharide B3-PS2 from Herba Scutellariae Barbatae could be a potential candidate in treating those complement-associated diseases.