兔VX2肺癌模型的建立及评价

Reproduction and evaluation of VX2 lung cancer model in rabbits

目的探讨肺穿刺注入组织块建立兔VX2肺癌模型的方法,并与细胞块悬液注入法及支气管注入法建立的模型进行比较。方法新西兰大白兔18只,随机分成3组,分别采用CT引导下经皮穿刺注射瘤组织块(A组,n=6)、细胞悬液(B组,n=6)及DSA引导下气管内瘤块注入的方法种植于肺,肿瘤种植后14、21和28dCT平扫观察肿瘤大小及CT值。结果A,B组成瘤率均为100%,成瘤时间14～21d,A组CT示L瘤体大小为(1.5±0.5)cm,CT值为(30±14)HU,肿瘤转移率为1/6,实验兔存活时间为(28±6)d。B组CT示肺癌大小为(1.0±0.5)cm,CT值为(29±16)HU,肿瘤转移率为4/6,实验兔存活时间为(16±5)d。两组存活时间,肿瘤转移率差异有统计学意义(P<0.05)。C组成瘤率为0。结论CT引导下瘤块穿刺法建立兔VX2肺癌模型具有方法简单,成功率高,动物损伤小,转移率低等优点。

Objective To explore the practicability of establishing lung cancer model through the plant with rabbit VX2 puncture, and compare it with another two models respectively injected through trachea and chest with cell suspension. Methods 18 big white rabbits of Newzealand were divided into 3 groups randomly and averagely. Rabbits in group A received percutaneous puncture through chest and injected with VX2 tumor tissue mass under the guide of CT; rabbits in group B accepted the same puncture and were injected with cell suspension; in addition, rabbits in group C were planted through the trachea with the lead of DSA. The diameter and the CT value of the tumor were measured 14, 21 and 28 days after the planted operation. Results the successful rates in group A and B were 100%, while no tumor were detected in group C, and the tumor can be identified during 14-21 days. The diameter was （1.5±0.5） cm in group A, and （1.0±0.5） cm in group B. At the same time the CT value was about （30±14） Hu in group A, and （29±16） Hu in group B. The rate of tumor metastasis in group A was 1/6, and the survival time was （28±6） d; Meanwhile, the rate in group B was 4/6, and the survival the time was （16±5） d. There was statistically significant in survival time and the rate of tumor metastasis between group A and B. Conclusion It is a convenient and effective way to establish the rabbit VX2 lung cancer by the tissue mass injection through chest with CT guidance.