浙江产温郁金挥发油的HPLC指纹图谱研究

HPLC Fingerprints of volatile oil of Curcuma wenyujin in Zhejiang Province

目的利用高效液相色谱建立温郁金挥发油的指纹图谱。方法采用Shimadzu VP-ODS C18色谱柱(250mm×4.6 mm,5μm),以乙腈-0.1磷酸溶液梯度洗脱,体积流量为1.0 mL/min,柱温30℃,检测波长210 nm。结果以=牛儿酮对照,通过对19批浙江不同产地温郁金样品测定,标定了14个共有峰,并通过"中药色谱指纹图谱相似度评价系统软件"和聚类分析等辅助手段,计算相似度等有关参数。结论该方法准确可靠,重现性好,为更好的控制温郁金内在质量提供了科学依据。

Objective To establish the chromatographic fingerprint of Curcuma wenyujin by HPLC. Methods The chromatographic fingerprint of C. wenyujin was established by HPLC with Shimadzu VPODS C18 column（250 mm×4.6 mm,5 μm）, acetonitrile-water （0. 1 phosphoric acid） gradient elution, the flow rate of 1.0 mL/min, column temperatures of 30 ℃, and detective wavelength at 210 nm. Results Taking germacrone as the reference peak, 14 common-peaks were selected as the fingerprint peaks. The similarities among samples of C. wenyujin among 19 approved samples and their standard chromatographic fingerprints were calculated with a software ＂Similarity Evaluation System for Chromatographic Finger- print of Chinese Materia Medica＂ published by GPC （Version 2004A） and cluster analysis. Conclusion This method with better accuracy and reproducibility could be used in the quality control of C. wenyujin.