正交设计优选卷柏中总黄酮的提取工艺研究

Optimization of extracting technology for flavonoids in Selaginella tamariscina with orthogonal design

目的优选卷柏中总黄酮的提取工艺。方法采用紫外分光光度法和高效液相色谱法分别测定总黄酮和阿曼托双黄酮。以提取量为考察指标，用正交设计方法优选最佳提取工艺。结果以总黄酮提取量为考察指标，其优选工艺为A1B2C2D2；以阿曼托双黄酮提取量为考察指标，其优选工艺为A1-3B1-3C2D2。综合考察采用A1B2C2D2为最佳提取工艺，即药材加10倍量的95％乙醇，回流提取2次，每次2h。结论优选得到的工艺稳定可行，可作为卷柏的提取工艺。

Objective To optimize the extraction technology for total flavonoids in Selaginella tarnariscina. Methods To determine the content of total flavonoids by UV and the content of index constituent of amentoflavone by HPLC. The optimum extraction condition was investigated by orthogonal design and the extraction quantity was regarded as the investigated index. Results The optimum extracting condition was A1 B2 C2 D2 with the extraction quantity of total flavonoids as the investigated index. The optimum extracting condition was A1-3B1-3C2D2 with the extraction quantity of amentoflavone as the investigated index. The optimum extracting condition was A1B2C2D2. That is adding ten times amount of 95% alcohol and refluxing twice, 2 h once. Conclusion The optimum technology is stable and feasible for the extraction of S. tarnariscina.