摘要
目的采用HPLC法对广东土牛膝进行指纹图谱研究,为广东土牛膝药材质量控制提供依据。方法实验采用Kromasil C18柱(250 mm×4.6 mm,5μm),以乙腈-水为流动相梯度洗脱,体积流量1.0 mL/min,柱温25℃,检测波长230 nm。结果建立广东土牛膝药材HPLC指纹图谱,确定13个共有峰,确定其相对保留时间和相对峰面积。结论在选定的条件下所建立的指纹图谱为广东土牛膝药材质量控制提供依据。
Objective To establish the HPI.C fingerprint of Eupatoriurn chinense. Methods The analysis was performed on a Kromasil C18 column （250 min×4.6 mm, 5 μm） with acetonitrile-water as mobile phase in a gradient mode. The flow rate was 1.0 mL/min. The column temperature was 25 ℃ and the detection wavelength was 230 nm. Results The fingerprint of E. chinense with common 13 peaks was established. The relative retention time and the ranges of relative area of the common peaks were determined. Conclusion The established fingerprint could be used for the quality control of E. chinense.
出处
《中草药》
CAS
CSCD
北大核心
2009年第6期964-967,共4页
Chinese Traditional and Herbal Drugs
