光动力疗法对S_(180)荷瘤小鼠肿瘤细胞损伤的红外光谱研究

Study of FTIR of mice′s tumor cell(S_(180)) damage by PDT

测定了光动力疗法对S180荷瘤小鼠肿瘤细胞损伤的红外光谱。结果表明,肿瘤细胞损伤后核酸、蛋白质、脂质的特征峰都有不同程度的改变,表现为峰值降低,频率发生蓝移或红移。结论:光动力作用肿瘤组织,可使肿瘤细胞的核酸、蛋白质、脂质的构型和构象发生变化。DNA单、双链发生断裂,蛋白质失去活性,细胞膜功能丧失。细胞的结构和功能被破坏,表现为细胞的凋亡和坏死。

In this paper, FTIR has been applied to the investigation of the tumor cell of mouse transplanted tissue injured by PDT （Photodynamic Therapy）. Experimental results show that the characteristic bands of nucleic acid, protein, and lipid changed to different extents, resulting in decreasing of peak and frequency shift to higher wave number or lower wave number. Conclusion ： the configuration and conformation of nucleic acid, protein, and lipid changed after PDT acted on tumor tissues. These caused that the DNA strand was induced a cleavage, protein lost activity, and cell membrane function declined. Moreover, the structure and functional of the cell were damaged,leading to cell apoptosis or death.