摘要
黄花蒿是中国传统使用的抗疟药,但由于同属中的茵陈蒿、青蒿两种植物在形态上与黄花蒿非常相似,导致某些地方常用茵陈蒿、青蒿植物代替黄花蒿使用。为了给蒿属药用植物的鉴定提供科学依据,采用叶绿体上psbA-trnH序列中的信息,运用物种特异的DNA序列信息位点区分黄花蒿和常见同属植物的混淆种茵陈蒿、青蒿。结果表明黄花蒿和茵陈蒿、青蒿植物之间psbA-trnH序列虽具有较高的相似性,但也有显著的特征性的不同,可以依靠它们各自特有的信息位点区分开来。该方法首次为蒿属药用植物的分子鉴定提供了可靠、简便的参考依据。
Artemisia annua L. is a traditional Chinese herbal medicine used in the treatment of malaria disease. But the dry samples of Artemisia capillaris Thunb. and Artemisia carvifolia Buch. are also used as A. annua in local areas. The adulterants are similar to A. annua and it is difficult to identify the botanical origin of these herbs. This research focused on analyzing the differences of psbA-trnH region sequences of A. annua, A. capillaris and A. carvifolia. The result showed that although the psbA-trnH regions among the three species are high similar; there are several character-sites for themselves, which can be used in discriminating them. It is the first time to use DNA technology in drug species＇ discrimination of Artemisia, which provided a reliable and simple reference for the authentication of them.
出处
《中国农学通报》
CSCD
北大核心
2009年第12期46-49,共4页
Chinese Agricultural Science Bulletin
基金
云南省教育厅科学研究基金项目(08Y0165)
