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CUEDC1的克隆表达及其对PRB和ERα转录活性的影响 被引量：3

Cloning of CUEDC1 and Effect on the Transactivity Mediated by PRB and ERα
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摘要 为了研究CUEDomain Containing1(CUEDC1)蛋白质功能,构建了pcDNA3.0-Flag-CUEDC1表达载体,并且成功表达Flag-CUEDC1蛋白质。通过双荧光报告系统,对比CUEDC1与CUEDC2对PRB和ERα转录活性的影响。其结果显示,CUEDC2能够抑制PRB和ERα转录活性,而CUEDC1对PRB和ERα转录活性的影响不显著。 In order to explore the function of CUEDC1, pcDNA3.0-Flag-CUEDC1 was constructed and Flag- CUEDC1 protein was obtained. Using Dual-Luciferase Reporter Assay System, the effects of CUEDC1 and CUEDC2 are detected on PRB or ERα mediated transactivity. The results demonstrated that CUEDC2 inhibits the transactivity of PRB and ERα, whereas CUEDC1 has no effect on transactivity mediated by PRB and ERα.
作者 穆蕊 赵洁 陈媛 张佩景 李慧艳 周涛 张学敏 李爱玲
机构地区 国家生物医学分析中心
出处 《科学技术与工程》 2009年第11期3041-3043,共3页 Science Technology and Engineering
基金 国家自然科学基金(30770471)资助
关键词 CUEDC1 CUEDC2 孕激素受体(PRB) 雌激素受体(ERα) 转录活性 CUEDC1 CUEDC2 PRB ERα transcriptional activity
分类号 Q579.13 [生物学—生物化学]
  • 相关文献

参考文献7

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同被引文献19

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科学技术与工程
2009年 第11期

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