佛手乙酸乙酯提取液对哮喘模型小鼠的抗炎作用

Effect of ethy1 acetate extract from bergamot in asthmatic mouse model

目的探讨佛手乙酸乙酯提取液(ethyl acetate extract from bergamo)对哮喘模型小鼠的抗炎作用。方法采用卵白蛋白(OVA)腹腔注射致敏和雾化激发的方法建立小鼠哮喘模型,用佛手乙酸乙酯提取液(10 g/kg)对哮喘小鼠进行干预,并以地塞米松作为阳性对照。比较观察各组小鼠外周血白细胞总数及分类计数,同时观察肺组织的病理改变。结果佛手乙酸乙酯提取液组和地塞米松组的外周血白细胞总数、嗜酸性粒细胞、淋巴细胞均显著低于哮喘模型组(P<0.05或P<0.01),而地塞米松组、佛手乙酸乙酯提取液组间上述指标无显著性差异(P>0.05)。病理改变:哮喘模型组小鼠肺组织可见支气管及伴行血管周围有大量炎性细胞(嗜酸性粒细胞、浆细胞、淋巴细胞等)浸润,肺间质及肺泡腔也可见炎性细胞浸润,气道上皮可见粘液分泌;而佛手乙酸乙酯提取液组和地塞米松组肺组织中炎性细胞浸润明显减少,气道上皮未见粘液分泌。结论佛手乙酸乙酯提取液能抑制哮喘模型小鼠嗜酸性粒细胞性炎症反应。

Objective To explore the effect of ethyl acetate extract from bergamot（EAEB） on inflammation leukocytes in asthmatic mouse model. Methods The asthmatic mouse model was established by sensitization of intraperitoneal injection and atomized stimulation with ovalbumin （OVA）. Some asthma model mice were intervened with EAEB （EAEB group） and others were positive control by receiving dexamethasone （ DXM ） treatment （ DXM group）. The number of leukocytes and eosinophils in peripheral blood was counted and pathological change of pulmonay tissue was examined. Results Compared with the asthmatic group,the number of lcukocytes, eosinophils and lymphocytes in the peripheral blood of EAEB group and DXM group were significantly lower （P 〈0.05 and P 〈0.01, respectively） , while no significant difference of these indices was found between the EAEB group and DXM group（P 〉0. 05 ）. Pathological examination of lung tissue of asthmatic group showed that large amount of inflammation cells （eosinopbils, plasmocytes and lymphocytes） were found surrounding the bronchius and vessels. Inflammation cell infiltration was also detected in pulmonary mesenchyme and alveolus and mucus secretion was found in airway epithelium. While compared with asthmatic group, the EAEB group and DXM group had less inflammation cells infiltration in lung tissue and no mucus secretion in airway epithelium. Conclusion EABA can suppress eosinophilic inflammation in asthmatic mice.