富硒蛹虫草的抗氧化活性研究

Research on Antioxidative Activity of Chrysalis Aweto Enriched in Selenium

采用现代分离技术从富硒蛹虫草中提取硒多糖,然后以苯酚-硫酸法、电感耦合等离子体-质谱(ICP-MS)法对硒多糖中多糖含量和硒含量进行测定;在Fenton体系的最佳实验条件下,研究了硒多糖对羟自由基的清除作用及硒多糖对支撑磷脂双层膜(Suppor-ted Bilayer Lipid Membranes,s-BLM)的保护作用。结果表明:硒多糖清除羟自由基的效果比与之相同浓度的无机硒、多糖都要明显。其中硒多糖对羟自由基的最高清除率可达38.02%,硒多糖具有很强的抗脂质过氧化、保护支撑磷脂双层膜的作用。

Se polysaccharide was extracted from chrysalis aweto enriched in Selenium by modern separation technique. The concentration of polysaccharides and selenium in Se polysaccharide was determined by Phenyl hydrate-sulfuric acid and ICP MS. The hydroxyl free radical scavenging of Sepolysaccharide and the protective effect of Selenium polysaccharide on s-BLM under optimal conditions of Fenton system were studied. The result indicated that the free radical scavenging of Se-polysaccharide was more obvious than that of the inorganic selenium and the polysaccharide under the same conditions; The highest clearance percentage of Se-polysaccharide was 38.02%, and Se-polysaccharide showed the ability to inhibit Lipid Peroxidation and the to proted s-BLM.