对氯苯基荧光酮与锌显色反应的研究及应用

Study on Chromogenic Reaction of Zinc with p-Chlorophenylfluorone and Its Application

在阳离子表面活性剂溴化十六烷基三甲胺（CTMAB）存在下，研究了对氯苯基荧光酮（P—CPF）与锌的显色反应条件和光度性质。在pH=8．4的Na2HPO4-KH2PO4缓冲溶液中，锌与P—CPF形成1：1的红色络合物，所形成的络合物的最大吸收波长位于568nm处，表观摩尔吸光系数为7．20×10^4L·mol^-1·cm^-1；红色络合物至少稳定10h以上，锌含量在0～2．40μg／5mL范围内符合比耳定律。拟定方法用于药物中微量锌的测定，结果满意。

In the presence of cationic surfactant cetyltrimethylammonium bromide（CTMAB）, reaction conditions and photometric properties of p-chlorophenylfluorone （p-CPF） with zinc were studied. The results showed that the molar ratio of zinc and p-CPF in the red complex was 1 ： 1, the maximum absorption wavelength of p-CPF-Zn-CTMAB complex was 568 nm and the apparent molar absorptivity was 7.20× 10^4 L.mo1^-1 .cm^-1 in the buffer solution （pH 8.4 of Na2HPO4-KH2PO4 ）. The red complex was stable for 10 hours at least. Beer＇s law was obeyed when the content of zinc was in the range of 0- 2.40 μg/5mL. The developed method has been applied to the determination of trace zinc in the medicine with satisfactory results.