纳米级小肽螯合铜对小白鼠生长性能和免疫器官的影响

Effect of nano-small peptide chelated copper on growth performance and immune organs of mouse

此试验的目的是探讨以硫酸铜、碱式氯化铜、蛋氨酸铜、纳米铜和纳米小肽螯合铜等不同铜源供给形式对小白鼠生长性能和免疫器官的影响。将120只18日龄小白鼠随机分为6个处理组,分别为Ⅰ(小肽+硫酸铜组)、Ⅱ(硫酸铜组)、Ⅲ(碱式氯化铜组)、Ⅳ(蛋氨酸铜)、Ⅴ(纳米铜组)和Ⅵ(纳米小肽螯合铜)等,每个处理组设4个重复,每个重复5只小白鼠。饲喂试验分0～5d、6～12d、13～19d、20～26d和27～33d五个阶段。其中0～5d为预试验期,后四个阶段为试验期。预试期和试验期各处理组采用相同的基础日粮。试验期Ⅰ～Ⅵ组饲粮铜的含量(以铜计)在6～12d、13～19d、20～26d和27～33d四个阶段分别为2.5mg、5mg、10mg及15mg。结果表明,纳米小肽螯合铜能有效促进小鼠增重。采食不同铜源日粮对小鼠免疫器官指数的影响随饲养日龄增大而减小,并随铜的供给量由低剂量变化到正常剂量而差异减小。

The purpose of this study was to examine influence of the different copper sources include copper sulfate, basic copper chloride, methionine copper, nanocopper and nano-small peptide chelated copper on growth performance and immune organs in mice. 120 18-day-old mice were randomly divided into six treatment groups, Ⅰ （small peptide＋copper sulfate）, Ⅱ （copper sulfate）, Ⅲ （basic copper chloride）, Ⅳ （methionine copper）, Ⅴ （nano-eopper） and Ⅵ （nano-small peptide chelated copper）. For each treatment with four duplicates, and each duplicate with five mice. Feeding trial was devided in to five phases from 0-5d, 6- 12d, 13 - 19d, 20- 26d and 27- 33d. The 0-5d phase is the pre-trial period. All the groups used the same basic diet. In the five trial phases, dietary copper content were supplied with 2.5mg, 5mg, 10mg and 15mg. The results showed that the nano-small peptide copper chelation is more effective to promote weight gain of mice. The influence of different copper source on the immune organ indexes of mice are reduced as the mice grow older, and the differences in immune organ indexes of mice taking different copper source rations are lessened with the copper content change from low level to normal level.