胶原修饰快速成形PLGA／TCP人工骨支架体外生物相容性研究

Biocompatibility of Rappid Promotyping PLGA/TCP Tissue Engineered Bone Scaffolds Coated with Collagen in vitro

探讨经Ⅰ型胶原修饰的快速成型(RP)聚乳酸-羟基乙酸/磷酸三钙(PLGA/TCP)支架的生物相容性,为进一步体内实验提供研究基础。以经Ⅰ型胶原修饰改性的PLGA/TCP支架作为实验组,未经胶原修饰的原始支架作为对照组,检测两组支架材料的亲水性。BMSCs接种两组支架,分别测定细胞黏附率、细胞增殖率。扫描电镜(SEM)观察细胞黏附形态,以检验支架材料的细胞相容性。结果表明胶原改性支架具有较高的亲水性,实验组细胞黏附率在接种4、8和12h者,明显优于对照组(P<0.05)。实验组在培养2、4、6和8d的细胞增殖率也明显高于对照组(P<0.05)。扫描电镜可见骨髓基质细胞在实验组支架上的增殖数量多于对照组。说明Ⅰ型胶原改性的PLGA/TCP支架具有良好的细胞生物相容性,可作为骨组织工程支架应用于骨缺损的修复研究。

The goal of present study is to investigate the biocompatibility of rapid prototyping （RP） polylactic glycolic acid/tricalcium phosphate （PLGA/TCP） scaffold coating with collagen Ⅰ and provide a basis for in vivo experiments. PLGA/TCP scaffold fabricated with RP technology and coated with collagen Ⅰ was used as experimental group and original scaffold without collagen Ⅰ was used as control group. After water absorption determined, BMSCs were seeded into both groups. Adhesion and proliferation ratio were examined. Morphology of BMSCs were observed under scanning electron microscope （SEM）. Higher hydrophilicity was performed in experimental group. Compared with control group, the Cell adhesion ratio was increased significantly in the experimental group at 4, 8, and 12 hour after the cell seeding（ P 〈 0.05）. As well, the cell proliferation of experimental group was demonstrated at higher level at 2, 4, 6, and 8 days （P 〈 0.05）. In addition, the quantity of BMSCs in experimental group was much more than control group under SEM. It is conclused this rapid prototyping PLGA/TCP scaffold coated with collagen Ⅰ possesses good cellular biocompatibility . It can be used as a scaffold to restore bone defects in bone tissue engineering.