2型糖尿病与代谢综合征患者血清脂联素水平及脂联素单核苷酸多态性45基因分析

Serum adiponectin level and adiponectin gene SNP45 in patients with type 2 diabetes mellitus and metabolic syndrome

目的探讨血清脂联素水平及脂联素基因单核苷酸多态性(SNP)45T→G与2型糖尿病(T2DM)及代谢综合征(MS)的关系。方法采用放射免疫法对MS组患者183例、T2DM组患者180例和健康人群(对照组)144例进行血清脂联素水平测定,并用Taq Man探针技术进行脂联素基因SNP45分析。结果T2DM组和MS组血清脂联素水平较对照组显著降低(7.17±3.43)mg/L,(6.81±2.99)mg/L vs(12.06±5.74)mg/L(均P<0.05);多元逐步回归分析显示:空腹胰岛素是影响MS组患者血清脂联素水平的独立相关因素。脂联素基因多态性分析:SNP45在T2DM组、MS组与对照组比较差异有统计学意义(P<0.05);等位基因分布频率提示G等位基因在T2DM组与MS组中多见(P<0.05);T2DM组、MS组中携带TG+GG型的患者血清脂联素水平(6.63±2.79)mg/L、(6.19±2.40)mg/L显著低于TT型的患者(9.17±4.47)mg/L、(8.20±3.88)mg/L(P<0.05)。结论低脂联素血症与T2DM、MS的发生发展有关,SNP45 G/G基因型可能是中国汉族人MS的易感基因。

Objective To investigate the association of serum adiponectin level and single nucleotide polymorphisms （SNP） 45T→G in the adiponectin gene with type 2 diabetes mellitus（T2DM） and metabolic syndrome （MS）. Methods Serum adiponectin levels of 183 patients with MS, 180 patients with T2DM（T2DM group） and 103 participants were assayed by RIA. Adiponectin gene SNP45 was analyzed by TaqMan technology. Results The serum adiponectin levels in T2DM group and MS group were significantly lower than that in control group （7.17±3.43） mg/L, （6.81±2.99） mg/L vs （12.06±5.74） mg/L （ P〈0.05）. Stepwise multiple regression analysis revealed that fasting serum insulin level was independently correlated with the serum adiponectin level in MS group. The distributions of SNP45 genotypes were more common in T2DM and MS groups than in control group （ P 〈0.05）. The analysis of allele frequency showed the major allele was G in T2DM patients and MS patients（ P〈0.05）. The patients with SNP45 TG or GG genotype in T2DM and MS groups had lower serum adiponectin level than those with TT genotype, （6.63±2.79） mg/L, （6.19±2.40） mg/L vs （9.17±4.47） mg/L, （8.20±3.88） mg/L（P 〈0.05）. Conclusion Hypoadiponectinemia is related to the development of T2DM and MS. SNP45 G/G genotype seems to be the suseeptive gene in MS patients of Chinese Han .