痛泻要方对腹泻型肠易激综合征Cajal间质细胞影响的研究

The Effect of Tongxie Yaofang Decoction on Cajal Cells in a Rat Model of D-IBS

目的:通过研究腹泻型肠易激综合征(D-IBS)大鼠结肠肌层Cajal间质细胞C-k it的表达,了解其在肠道内分布、数量等变化,以探讨痛泻要方治疗D—IBS的可能机制。方法:36只W istar大鼠随机分为3组,正常组12只、模型组12只、中药组12只。采用番泻叶+束缚应激刺激造成腹泻型肠易激综合征动物模型,正常组及模型组予生理盐水灌胃,中药组予痛泻要方颗粒剂灌胃治疗。应用透射电镜检查结肠Cajal细胞超微结构变化;免疫组化染色法检测结肠C-k it表达阳性细胞数量表达。结果:D—IBS大鼠结肠C-k it阳性细胞的分布面积及数目增多,经予痛泻要方干预后C-k it阳性细胞变化不明显,统计学上无差异(P>0.05)。结论:D-IBS大鼠结肠平滑肌ICC增多可能是D-IBS发病机制之一,痛泻要方对大鼠结肠Cajal间质细胞无明显作用。

Objective：We diseusss the effect of Tongxie Yaofang decoction on Cajal cells in a rat model of diarrhea-predominant irritable bowel syndrome （ D - IBS ） through research the expression of C - kit about intestinal cells of Cajal （ICC） in the intestine of rat with D -IBS as well as through invesatigate the distribution and variation of ICC and the role of ICC in the pathophysiology of D - IBS. Methods ： Thirsty - six Wistar mouse were randomly divided into three groups, such as ：group1（ normal group）, group 2 （blank group）, group 3 （tradition Chinese medicine group）. The diarrhea-predom- inate IBS model was made by Fanxieye and bondage stress stimulus. Normal group and blank group received a daily intra- gastric administration of 0.9% NaCl,while the other group received a daily intragastric administration of Tongrde Yaofang decoction. The ultrastructure of intetinal cells of Cajal was detected with transmission electron microscope. Immunehistochemistry stain was used to assay the square and expression of C - kit cells. Results ： Compared with the normal group, the square and expression of C - kit cells are on the increase in a rat model of D - IBS. After treated with Tongxie yaofang decoction ,the ehgnges of C - kit cells is not distinct. There is no significant difference between blank group and TCM group. Conclusions： The increment of ICC in a rat model of D - IBS maybe is a cause of D - IBS. Tongxie yaofang decoction is no effect on ICC.