不同炮制方法对延胡索中有效成分含量的影响

Effect of Different Processes on the Contents of Active Ingredients in Corydalis

目的比较不同炮制方法对延胡索中3种有效成分含量的影响。方法将鲜延胡索、水煮延胡索和市售的延胡索药材分别经醋煮、醋炙和酒炙后,分别采用水超声法和甲醇热回流法进行提取,采用反相高效液相色谱法测定3种有效成分的含量。色谱柱为AgilentTC-C18(250mm×4.6mm,5μm),流动相:乙腈-0.1%磷酸溶液(三乙胺调pH5.3)为28∶72,流速:1mL/min,柱温:35℃,检测波长:280nm。结果延胡索经炮制后水提液中生物碱含量均有所提高,醇提液中生物碱含量也有上升的趋势;延胡索鲜品直接经醋炙、醋煮、酒炙处理后,去氢紫堇碱含量较高,延胡索在产地水煮后延胡索乙素含量较高。结论本研究建立了同时检测延胡索中3种有效成分的检测方法,该方法简便、可靠、重复性好。根据临床需要,延胡索炮制工艺可采用鲜品水煮后再炮制或产地加工和炮制一体化的两种操作方式。

Objective To explore the effect of different processes on the contents of active ingredients in Rhizoma Corydalis. Methods Processes of vinegar-boiling, vinegar-frying and alcohol-frying were used to fresh corydalis, boiled corydalis and purchased corydalis. Then the method of extraction by ultrasound with water and by reflux with methanol were used to extract the sample. The contents of Protopine, Tetrahydropalmatine and Dehydrocorydaline in corydalis was determined by RP-HPLC. Agilent TC-CI8 （25 mm × 4.6 mm, 5μm） column was used at 35℃, with acetonitrile-0.1% phosphonic acid （pH 5.3 with triethylamine） （28 ： 72） as mobile phase. The flow rate was 1 mL/min and detection wavelength was set at 280 nm. Results In the sample extracted by water, the contents of active ingredients in processed products were higher than in corydalis. And in the sample extracted by methanol, it also has the tendency that the contents of active ingredients in processed products were higher than in corydalis. The content of Dehydrocorydaline in fresh corydalis which was processed by vinegar-boiling, vinegar-frying and alcoholfrying was high. The content of Tetrahydropalmatine in purchased corydalis which was processed by water-boiling at production place was high. Conclusion The method of determining active ingredients in Rhizoma Corydalis was established. It was convenient and the result was accurate. The process technology that fresh corydalis was boiled by water and then processed or fresh corydalis was processed in production place can be used.