不育症患者精液支原体检测及耐药性分析

Identification and Drug Resistance of Mycoplasma from Semen of infertility Men

目的探讨男性不育症患者精液支原体感染及药敏情况。方法应用培养法对男性不育症患者精液进行支原体检测,同时测定其对抗菌药物的耐药性。结果267份标本中支原体感染率为46.07%,其中单纯解脲脲支原体(Uu)感染率为41.57%(11例)、单纯人支原体(Mh)感染率为1.50%(4例),Uu+Mh混合感染率为3.00%(8例);9种抗菌药物药敏试验结果显示,支原体对米诺环素、多西环素和交沙霉素的敏感率最高,阿奇霉素和罗红霉素次之;耐药率最高的是氧氟沙星和克林霉素。结论男性不育症患者精液支原体的感染已相当普遍,临床应引起高度重视并加强支原体培养和药敏试验,根据药敏试验结果选择用药,从而最大限度地控制耐药菌株的产生。

OBJECTIVE To study infection and drug resistance of mycoplasma from semen of infertility men. METHODS Mycoplasma from semen of infertility men was identified by cultivation, and the sensitivities to drugs were also performed. RESULTS In 267 cases the positive rate of mycoplasma was 46.07%. Simple infection of Ureaplasma urealyticurn （Uu） accounted for 41.57% （11）, and Mycoplasrna horninis （Mh） 1.50% （4）, and the mixed 3.00% （8）. The result of drug sensitive test showed that sensitivities of mycoplasma to minocycline, doxycycline and josamycin were the highest, and then were roxithromycin and azithromycin. The drug resistance of mycoplasma to ofloxacin and clindamycin was the highest. CONCLUSIONS The infectious rate of mycoplasma from semen of infertility men is on big rise. It is important to culture and test the drug sensitivities of mycoplasma to use drugs rationally.